Peroxicats: PEROXidases as bioCATalystS
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CEPI - Confederation of European Paper Industries
Cefic - The European Chemical Industry Council
Industrial use of enzymes
Enzymes catalyze a huge amount of chemical reactions in living organisms, which take place under mild conditions compatible with life, and with exquisite substrate specificity, which permits the simultaneous assemblage of the extraordinarily complex catabolic and anabolic routes that characterize life. Nowadays, the availability of genetic engineering tools permits large-scale production of enzymes and other proteins at low cost by isolating (or synthesizing) the corresponding genes (or cDNA) and introducing them into adequate expression hosts (often filamentous fungi) after their cloning in expression vectors. Simultaneously, protein engineering using rational and non-rational designs permits to adapt these enzymes to the industrial application conditions, and to increase their expression yields.

This situation represented a real breakthrough for industrial biotechnology enabling the production of commercial enzymes at very low costs. The enzymatic biocatalysts obtained are often very competitive and more environmentally-friendly than the corresponding chemical reagents, at the same time that their substrate specificity permits to obtain specific products (including stereo-specific transformations) that are difficult and/or very expensive to obtain by pure chemical synthesis. In addition to their traditional uses, e.g. in the manufacture of some food products and in diverse microbiological transformations (e.g. fermentation processes), the last decades have seen how enzymes entered different industrial sectors, such as detergents, food and beverages, textiles, pulp and paper, leather and personal care. Moreover, enzymes have recently started to occupy an important position in the fuel markets, and are slowly entering the market of fine chemicals.
eu Official website of peroxicats [Peroxidases as biocatalysts]. Novel and more robust fungal peroxidases as industrial biocatalysts. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: KBBE-2010-4-265397. © Peroxicats 2011. Developed by Shunet. This site is optimized for the following versions and browsers: Internet Explorer 8 or higher, Firefox 3.6 or higher, Safari 5 or higher, Google Chrome 10 or higher and Opera 10.10 or higher.

Chromatographic analysis of reaction products
Chromatographic analysis of reaction products.